Spinning-disk

Photon Microscopy
Confocal & Spinning-Disk
Photomanipulation
Live acquisitions - Photomanipulation
spinning-disk

Spinning Disk Confocal Microscope


Inverted NIKON Ti-E microscope driven by Metamorph and ILAS2 software
    
Spinning Disk CSU-X1 (YOKOGAWA)


Lasers   

  • 405 nm
  • 491 nm
  • 561 nm
  • 633 nm

 

Emission filter for acquisition

 

Cube Filter for observation
    

  • Dapi Ex: (BP377/50nm) DM : 409
  • FITC Ex: (BP482/35nm) Em : 536/40nm
  • RED Ex: (BP 510-560) Em : (LP 590)


Objectives
    

  • 10X PLAN FLUOR - ON 0,3
  • 60X PLAN APO- ON 1,4 - oil
  • 100X PLAN APO ON 1,4 -oil


motorized XY and Z stage

temperature  control (LIS)


Camera sCMOS ORCA Flash 4.0 (Hamamatsu) - 2048x2048 - 6,5 µm

Applications    

Fast confocal imaging
Videomicroscopy
FRAP