Leica DMIRB

Photon Microscopy
Widefield
Calcium Imaging
Inverted widefield microscope with Calcium / pH imaging capabilities
DMIRB

Inverted Leica DMIRB

Metamorph and Metafluor softwares

Motorized XY Stage
Piezo for Z acquisition (single objective)

 

Illumination

  • Lambda DG-4 Xenon lamp
  • 340 excitation filter
  • 376 excitation filter
  • 480 excitation filter
  • 525 excitation filter

 

Objectives

  • 63X/1,40 HCX PL APO Oil
  • 40X/1.35 UApo N340 (Olympus)
  • 25X/0.75 PL FLUOTAR Oil

 

Detection

  • Princeton Instruments CoolSnapHQ Camera (1392x1040 - 6,45 µm)
  • GFP emission filter
  • TRITC emission filter
  • Cyanine 5 emission filter
  • SNARF (pH imaging)
  • FURA (Calcium imaging)

 

Environment

  • CO2 and temperature control